Coding

Part:BBa_K4212007:Design

Designed by: Shirin Bamezai   Group: iGEM22_Imperial_College_London   (2022-09-29)


Cas9


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3823
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3823
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3823
    Illegal BglII site found at 1188
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3823
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3823
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimised for B. subtilis, with recognition sites for BbsI, BsaI and BsmbI Type IIS enzymes removed to enable correct assembly in Golden Gate system.


Source

Streptococcus pyogenes

References